Nucleic Acid Amplification using Recombinase Polymerase: Enzymatic Approach
نویسندگان
چکیده
منابع مشابه
Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA).
For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA). The system consists of a novel, foil-based centrifugal microfluidic cartridge including prestored liquid and dry reagents, and a commercially available centrifugal analyzer for i...
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Absolute, precise quantification methods expand the scope of nucleic acids research and have many practical applications. Digital polymerase chain reaction (dPCR) is a powerful method for nucleic acid detection and absolute quantification. However, it requires thermal cycling and accurate temperature control, which are difficult in resource-limited conditions. Accordingly, isothermal methods, s...
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Numerous reports have been published on the properties and reaction mechanism of the highly purified enzyme preparations that catalyze the deoxyribonucleic acid-directed synthesis of ribonucleic acid (l-6). The various RNA polymerases tudied, although differing in some respects, show a remarkable similarity in their cofactor requirements, reaction kinetics, physical properties, and facility to ...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology & Diagnosis
سال: 2017
ISSN: 2161-0703
DOI: 10.4172/2161-0703.1000250